Nanotechnology Sterilization in Dentistry

Abstract

Purpose: The goal of this research was to evaluate the effectiveness of the active Nbelyax cold sterilant (Éviter™)
with nanotechnology in three different bacterial strains of great importance in dentistry: E. faecalis, S. aureus
and Neisseria sp. Materials and Method: An experimental, observational and qualitative study using three
different bacterial strains was performed. The sterilant product was prepared at 1: 20 dilution. Then, in a 250
mL Erlenmeyer flask were poured 99 mL of the sterilant prepared, each bacterial strain was inoculated in same
flask, allowing two different contact times: 5 and 30 minutes. Subsequently, an aliquot of 0.1 mL in 18 mL
of trypticase soy per petri plaque was done using pour plate technique and incubated 48 hours at 37 °C. The
experiment was done by duplicate. The SPSS statistical program version 20.0 was used, applying Mcnemar
statistical test to analyze the results. Results: This research showed that after 5 minutes of contact with the
cold sterilant Éviter, E. faecalis and S. aureus strains had bacterial growth, however Neisseria sp. did not have
bacterial colonies growth. The three bacterial strains after 30 minutes of contact did not show colonies growth.
McNemar test did not show significant statistical differences (p≥0.05). Conclusions: With the sample and ramimethodology
used, we observed that the recommended contact time of the cold sterilant Éviter product with
nanotechnology to eliminate the microorganism tasted was 30 minutes.