Saliva clinical diagnosis. Review
DOI:
https://doi.org/10.59334/ROV.v1i26.226Keywords:
Biomarkers, pharmacotherapy, immunohistochemistry, FRET, gelatin zymography, DNA microarraysAbstract
The dentist is called to become an important element in the development of studies phenotyping of populations to advance the implementation of personalized medicine and the reason is because saliva, which besides being a prerequisite for the preservation of oral functions fluid , is emerging with important qualities for clinical diagnosis with genetic basis. With the development of powerful laboratory techniques, such as immunoassay, electrophoresis, fluorescence, chromatography (HPLC and GC), mass spectrometry (MS), polymerase chain reaction (PCR) and genetic testing phenotyping, it has been correlate the presence of certain biomarkers of tissue damage in saliva levels of these species in blood. These biomarkers are signs of tissue damage or agency responses to these injuries, when not even be seen clinical evidence of it.
This application saliva attaches special importance with regard to clinical diagnosis. The study of oral biomarkers has joined, more recently, the development of protocols for obtaining genomic DNA from saliva that allows for viability in sample collection, preservation of the same and facilities during transport, conduct population studies to determine the function of certain genes that are related to biotransformation of drugs, a major cause of differences among individuals to drug treatments. Knowledge of polymorphisms in genes that express enzymes that metabolize specific drugs can make changes or adjustments active principles in doses of individual treatments, which is the goal of personalized medicine or pharmacogenetics.
These studies also provide insight into the genetic predisposition of populations to the development of certain diseases, both oral and systemic, which favors the establishment of new policies in the prophylaxis and preventive medicine. The use of saliva for this purpose opens new perspectives in care dental and requires interdisciplinary efforts and cooperation in the health team.
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References
Abraham, J.E. et ál. (2012). Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping. https://doi.org/10.1186/1755-8794-5-19
Bahlo M. et ál. (2010). Saliva-derived DNA performs well in large-scale, high-density single-nucleotide polymorphism microarray studies. Cancer Epidemiol Biomarkers & Prevention. 19(3): 794-798. https://doi.org/10.1158/1055-9965.EPI-09-0812
Baró, L. et ál. (2001). Disponible en: http://farmacia.ugr.es/ars/pdf/222.pdf
Barriga, A. G, y Hernández, S. E. (2016). Utilidad de las muestras de saliva en el diagnóstico por el laboratorio. Rev. Latinoam Patol Clin Med Lab. 63(1): 13-18. Versión electrónica disponible en: http://www.medigraphic.org.mx/
Baró, L. et ál. (2001). Disponible en: http://farmacia.ugr.es/ars/pdf/222.pdf
Baynes, J. N. y Dominiczak, M. (2015). Bioquímica médica. Cuarta edición. Elsevier Saunders. España.
Buys, Diego L. et ál. (2007). Interpretación básica de inmunohistoquímica. Características generales de diversos anticuerpos y su localización celular y sub-celular. Patología. Revista Latinoamericana. 45(3):126-140.
C. A. Christianson, B. L. Fitzsimmons, J. H. Shim et ál. (2012). Spinal matrix metalloproteinase 3 mediates inflammatory hyperalgesia via a TNF dependent mechanism. Neuroscience 200 199-210. https://doi.org/10.1016/j.neuroscience.2011.10.019
Cajaraville, P. M. Disponible en: http://www.ehu.eus/europeanclass2003/miren1.pdf
Castrillón LE, Palma A, Macín S. (2011). Papel de la lactoferrina en enfermedades periodontales. Revista Odontológica Mexicana; 15(4): 231 – 238. https://doi.org/10.22201/fo.1870199xp.2011.15.4.27869
Devlin, M. T. (2015). Bioquímica. Vol. I y II. 4ta edición. Editorial Reverte.
Doménech Berrozpe, José et ál. Editores (2013). Tratado general de Biofarmacia y Farmacocinética. Vol. I. LADME. Análisis farmacocinético. Biodisponibilidad y bioequivalencia. Editorial Síntesis. España.
Gutiérrez, G.R. (2004). Farmacogenética: medicina personalizada. Revista Cubana de Farmacia. Vol. 38(3). Set-Oct.
Hansen, T.V. et ál. (2007). Collection of blood, saliva, and buccal cell samples in a pilot study on the Danish nurse cohort: comparison of the response rate and quality of genomic DNA. Cancer Epidemiol Biomarkers & Prevention. 2072-2076. https://doi.org/10.1158/1055-9965.EPI-07-0611
Hasan M, Siegmund y W. Oswald S. (2016). Rapid LC-MS/MS: method for the determination of 4-hydroxycho-lesterol/cholesterol ratio in serum as endogenous biomarker for CYP3A activity in human and foals. J Chromatogr B Analyt Technol Biomed Life Sci. Aug 5; 1033-1034:193-199. https://doi.org/10.1016/j.jchromb.2016.08.006
Hernández, A. A. y Aranzazú, G. C. (2012). Características y propiedades físico-químicas de la saliva: una revisión. Rev. UstaSalud. 11(101-111). https://doi.org/10.15332/us.v11i2.1123
Hernández, A. J. L. (2012). Genética clínica. Tema selecto de genética VI. Genética y Bioética. Editorial El Manual Moderno. 1ra Edición. México, DF.
James, C, et ál. (2011). Human genomic DNA content of saliva samples collected with the Oragene® self-collection kit. DNA Genotek. PD-WP-011 Issue 4/11.
Leite, A. F. et ál. (2016). Immunoexpression of cleaved caspase-3 shows lower apoptotic area indices in lip carcinomas than in intraoral cancer. J Appl Oral Sci. Jul-Aug. 24(4):359-365. https://doi.org/10.1590/1678-775720160156
Lieberman, Michael y Marks, A. D. (2013). Bioquímica médica básica: un enfoque clínico. 4ta edición. Lippincott Williams & Wilkins.
Mathews, C. K. et ál. (2013). Bioquímica. 4ta edición. Editorial Pearson.
Murray, R. K. et ál. (2015). HARPER. Bioquímica ilustrada. 29a edición. McGraw Hill.
Nelson, D. L. y M. M. Cox (2014). Lheninger. Principios de Bioquímica. 6ta edición. Editorial Omega.
Novoa-Herrán, Sandra S. (2011). IGF-II Stimulates MMP-9 and MMP-2 Activity in a Human Trophoblast Model. Acta Biologica Colombiana 16(1):121-132.
Roma, Mateo C. (2016). La epigenética. Editorial La Catarata. España. 128 pp.
SEBIA (2015). Electroforesis en gel de agarosa. Hydragel 7 Protein(E). Ref. 4100.
Stryer, Lubert (2015). Bioquímica con aplicaciones clínicas. Vol. I y II. 7ma edición. Editorial Reverte.
Todorovic, Dozic I, et ál. (2006). Enzimas salivales y enfermedad periodontal. Med Oral Patol Oral Cir Bucal 2012; 11:115 - 119.
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